12/8/2023 0 Comments Amp stock on plates c.elegans![]() Modulation of aging profiles in isogenic populations of Caenorhabditis elegans by bacteria causing different extrinsic mortality rates. Demography of dietary restriction and death in Drosophila. Age-specific demographic profiles of longevity mutants in Caenorhabditis elegans show segmental effects. Johnson, T.E., Wu, D., Tedesco, P., Dames, S. Two-parameter logistic and Weibull equations provide better fits to survival data from isogenic populations of Caenorhabditis elegans in axenic culture than does the Gompertz model. Biodemographic trajectories of longevity. Mortality dynamics of density in the Mediterranean fruit fly. Demography of genotypes: failure of the limited life-span paradigm in Drosophila melanogaster. Analysis of aging in Caenorhabditis elegans. Measuring Caenorhabditis elegans life span on solid media. daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. elegans mutant that lives twice as long as wild type. Kenyon, C., Chang, J., Gensch, E., Rudner, A. A mutation in the age-1 gene in Caenorhabditis elegans lengthens life and reduces hermaphrodite fertility. What accounts for the wide variation in life span of genetically identical organisms reared in a constant environment? Mech. Our approach permits rapid, detailed reverse-genetic and chemical screens for effects on survival and enables quantitative investigations into the statistical structure of aging. The automated method generates a permanent visual record of individual deaths from which survival curves are constructed and validated, producing data consistent with results from the manual method of survival curve acquisition for several mutants in both standard and stressful environments. To enable the rapid acquisition of survival curves at an arbitrary statistical resolution, we developed a scalable imaging and analysis platform to observe nematodes over multiple weeks across square meters of agar surface at 8-μm resolution. Indeed, survival assays in Caenorhabditis elegans have provided critical insights into evolutionarily conserved determinants of aging. The actions of molecular mechanisms on lifespan are therefore visible only through their statistical effects on populations. Because lifespan results from complex interactions between genetic, environmental and stochastic factors, it varies widely even among isogenic individuals. The measurement of lifespan pervades aging research.
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